Journal: Molecular cell

Article Title: Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency

doi: 10.1016/j.molcel.2021.06.011

Figure Lengend Snippet: (A) Cell survival assays for T2, BR5, and BR5-R1 (BR5-derived PARPi-resistant cells) cells under increasing concentrations of olaparib.

Article Snippet: T2, BR5, BR5-R1 cell lines were cultured in DMEM (CORNING cellgro, 15-017-CV) with 10% FBS, penicillin and streptomycin (100 U/ml each), and 1% L-glutamine ( Yazinski et al., 2017 ).

Techniques: Derivative Assay

Journal: The Journal of Experimental Medicine

Article Title: Efficient Generation of a Hepatitis B Virus Cytotoxic T Lymphocyte Epitope Requires the Structural Features of Immunoproteasomes

doi:

Figure Lengend Snippet: Time-dependent generation of a potential precursor peptide HBcAg141-162 and of HBcAg141-151. HBcAg131-162 was digested for 0.5, 1, 2, 4, 8, and 24 h with 20S proteasome isolated from T2 cells and T2 LMP2+7 transfectant cells. Cleavage products were separated by HPLC as described in Materials and Methods but analyzed on-line by an ion trap mass spectrometer (ThermoQuest) equipped with an electrospray ion source, which results in increased sensitivity. Time course of the potential precursor peptide HBcAg141-162, which is generated by a single cleavage after Leu140 (A), and of the antigenic HBcAg141-151 (B) is depicted. Plotted values were confirmed by repeated analysis of the same digests.

Article Snippet: C4 (BALB/c) mouse fibroblast cells were cultured in Iscove's MEM (Biochrom), and the human cervical carcinoma cell line HeLa, the human lymphoblastoid cell line T2 , and T2 transfectant cell lines were cultured in RPMI (Biochrom).

Techniques: Isolation, Transfection, Mass Spectrometry, Generated

Journal: The Journal of Experimental Medicine

Article Title: Efficient Generation of a Hepatitis B Virus Cytotoxic T Lymphocyte Epitope Requires the Structural Features of Immunoproteasomes

doi:

Figure Lengend Snippet: Incorporation of the LMP2 and LMP7 subunits in 20S proteasomes of T2 transfectant lines. 500 ng (A) or 150 ng (B) of purified 20S proteasomes was separated by SDS-PAGE and probed with mouse LMP2–specific (A) or mouse LMP7–specific (B) polyclonal rabbit antisera. Molecular mass markers (kD) are shown at left.

Article Snippet: C4 (BALB/c) mouse fibroblast cells were cultured in Iscove's MEM (Biochrom), and the human cervical carcinoma cell line HeLa, the human lymphoblastoid cell line T2 , and T2 transfectant cell lines were cultured in RPMI (Biochrom).

Techniques: Transfection, Purification, SDS Page

Journal: The Journal of Experimental Medicine

Article Title: Efficient Generation of a Hepatitis B Virus Cytotoxic T Lymphocyte Epitope Requires the Structural Features of Immunoproteasomes

doi:

Figure Lengend Snippet: Kinetic analysis of the production of HBcAg141-151 and of related peptide fragments. HBcAg131-162 was digested for 4, 8, or 24 h with 20S proteasomes isolated from T2 LMP2+7 (A) and T2 LMP2+7 T1A transfectant cells (B) or mock-digested (C). Cleavage products were analyzed as described in Materials and Methods. Relative abundances of 11 selected peptides are plotted. Shown data were reproduced in a second, independent experiment.

Article Snippet: C4 (BALB/c) mouse fibroblast cells were cultured in Iscove's MEM (Biochrom), and the human cervical carcinoma cell line HeLa, the human lymphoblastoid cell line T2 , and T2 transfectant cell lines were cultured in RPMI (Biochrom).

Techniques: Isolation, Transfection